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KMID : 0921620110410010055
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Journal of Bacteriology and Virology
2011 Volume.41 No. 1 p.55 ~ p.61
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Recovery and Adsorption Rate of Murine Norovirus Using NanoCeram® Filters
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Kim Yun-Hee
Jang Seok-Jea
Park Ji-Youn
Oh Jung-Hwan
Kim Geun-Su
Kim Tae-Seung
Kwon Oh-Sang
Han Jin-Seok
Jheong Won-Hwa
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Abstract
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This study investigated the recovery and absorption rates of murine norovirus, a surrogate for human norovirus, by using NanoCeram® filters which served as a tool for recovering viruses. In the study, two types of NanoCeram® filters were employed: one was a cartridge type and the other was a disc type (φ 47 mm) whose surface area is 75 times smaller than the cartridge type. The analytical method was the real-time reverse transcription-polymerase chain reaction (RT-PCR). The study found that the average recovery rates of the cartridge type and the disc type were 30.9% and 29.5% respectively. Since these two rates were very close to each other, the adsorption rate of the cartridge type could be predicted with the disc type. Analyzing recovery and absorption rates of the disc type based on different filtered volumes showed that when the volume increased from 0.5 L to 20 L, the average recovery rate rose from 14.78% to 30.41 %, while the average absorption rate dropped from 56.33% to 10.48%. The increase in turbidity from less than 1 NTU to less than 3 NTU raised the average recovery rate from 47.23% to 82.84%.
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KEYWORD
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NanoCeram® filter, Norovirus, Murine norovirus, Recovery, Adsorption.
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